Composite

Part:BBa_K2057005

Designed by: José Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


Expression of gfp under the regulation of promoter ChiA74

We made this composite part to check the functionality of the promoter chiA74 in Escherichia coli. Biobrick BBa_K2057000 (promoter chiA74) includes the promoter sequence and the ribosome binding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1218


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biologyEscherichia coli